Skip to main content
NSF NEON | Open Data to Understand our Ecosystems logo

Main navigation

  • About Us
    • Overview
      • Spatial and Temporal Design
      • History
      • Management
    • Advisory Groups
      • Advisory Committee: STEAC
      • Technical Working Groups (TWGs)
    • FAQ
    • Contact Us
      • Field Offices
    • User Accounts
    • Staff

    About Us

  • Data & Samples
    • Data Portal
      • Explore Data Products
      • Spatial Data & Maps
      • Document Library
      • API & GraphQL
      • Prototype Data
        • Prototype Data Ingest
      • External Lab Data Ingest (restricted)
    • Samples & Specimens
      • Discover and Use NEON Samples
        • Sample Types
        • Sample Repositories
        • Sample Explorer
        • Megapit and Distributed Initial Characterization Soil Archives
        • Excess Samples
      • Sample Processing
      • Sample Quality
      • Taxonomic Lists
    • Collection Methods
      • Protocols & Standardized Methods
      • AIrborne Remote Sensing
        • Flight Box Design
        • Flight Schedules and Coverage
        • Daily Flight Reports
        • Camera
        • Imaging Spectrometer
        • Lidar
      • Automated Instruments
        • Site Level Sampling Design
        • Sensor Collection Frequency
        • Instrumented Collection Types
          • Meteorology
          • Phenocams
          • Soil Sensors
          • Ground Water
          • Surface Water
      • Observational Sampling
        • Site Level Sampling Design
        • Sampling Schedules
        • Observation Types
          • Aquatic Organisms
            • Aquatic Microbes
            • Fish
            • Macroinvertebrates & Zooplankton
            • Periphyton, Phytoplankton, and Aquatic Plants
          • Terrestrial Organisms
            • Birds
            • Ground Beetles
            • Mosquitoes
            • Small Mammals
            • Soil Microbes
            • Terrestrial Plants
            • Ticks
          • Hydrology & Geomorphology
            • Discharge
            • Geomorphology
          • Biogeochemistry
          • DNA Sequences
          • Pathogens
          • Sediments
          • Soils
    • Data Policies & Citation Guidelines
    • Data Notifications
    • Data Management
      • Data Availability
      • Data Formats and Conventions
      • Data Processing
      • Data Quality
      • Data Product Revisions and Releases
      • Externally Hosted Data

    Data & Samples

  • Field Sites
    • About Field Sites and Domains
    • Explore Field Sites

    Field Sites

  • Impact
    • Observatory Blog
    • Case Studies
    • Spotlights
    • Papers & Publications
    • Newsroom
      • NEON in the News
      • Newsletter Archive

    Impact

  • Resources
    • Documents and Communication Resources
      • Papers & Publications
      • Document Library
    • Code Hub
      • Code Resources Guidelines
      • Code Resources Submission
      • NEON's GitHub Organization Homepage
    • Learning Hub
      • Science Videos
      • Tutorials
      • Workshops & Courses
      • Teaching Modules
      • Faculty Mentoring Networks
      • Data Education Fellows
    • Research Support and Assignable Assets
      • Field Site Coordination
      • Letters of Support
      • Mobile Deployment Platforms
      • Permits and Permissions
      • AOP Flight Campaigns
      • Excess Samples
    • Community Forum

    Resources

  • Get Involved
    • Advisory Groups
    • Upcoming Events
    • Past Events
    • Community Engagement
    • Work Opportunities
      • Careers
      • Seasonal Fieldwork
      • Postdoctoral Fellows
      • Internships
        • Intern Alumni
    • Partners

    Get Involved

  • My Account
  • Search

Search

Discover and Use NEON Samples

  • Sample Types
  • Sample Repositories
  • Sample Explorer
  • Megapit and Distributed Initial Characterization Soil Archives
  • Excess Samples

Megapit and Distributed Initial Characterization Soil Archives

Soil samples

Terrestrial soil samples

The Megapit and Distributed Initial Characterization Soil Archives provide access to archived soils collected at the time of NEON site construction. Additional archived soils from ongoing sampling activities can be requested through the NEON Biorepository.

Megapit Soil Samples

At all terrestrial sites NEON collected soil from each horizon of a single temporary soil pit, called the megapit, to characterize and archive the soil conditions at the time of site construction. The pit location was selected to be in the locally dominant soil type surrounding the NEON tower based on available soil maps, accessible by a small excavator, and representative of the soil sensor locations. Samples were collected to 2 m at most sites because that depth range contains the vast majority of plant roots and soil organisms and is the location of most biogeochemical processes that influence, and are influenced by, the aboveground part of the ecosystem. The Megapit Archive is curated at the NEON program headquarters in Boulder, CO. Air-dried soil from each horizon is archived and available for request. 

Soil samples

Soil samples

Initial Characterization Soil Samples (ICS)

This archive is a joint project of Battelle/NEON and the University of Michigan. During initial operations, NEON contracted with the U.S. Department of Agriculture - Natural Resources Conservation Service (USDA-NRCS) to describe, sample, and analyze soils across NEON sites to a depth of up to 1 m. This effort included multiple components of the NEON design, including tower and distributed plots, and resulted in two data products now available from NEON in addition to the soil archive. The central goal of this campaign was the initial characterization of soils and their properties from all major soil types at each NEON site. Of the 3,246 soil horizons characterized from 46 NEON sites during the USDA-NRCS initial soil characterization campaign, ~2,055 samples from 33 sites possessed sufficient material for archive in the University of Michigan Biological Station - Sample Archive Facility in Ehlers (UMBS-SAFE), a community-accessible soil archive established with NSF support in 2016-18. Subsamples of these well-characterized, air-dried samples are now available to researchers who complete a request and review process, described below.
 

A comparison of Megapit vs. Initial Characterization Soils
  Megapit Soil Initial Characterization Soil
Horizons Multiple Multiple
Processing Air-dried, mineral soil sieved to ≤ 2 mm

Air-dried

Storage Conditions Ambient temperature, glass jars Ambient temperature
Volume/Mass Stored 1.2 or 3.6 kg per horizon Varies; typically 25-100 g for O horizons; 100-400 g for mineral horizons
Maximum Depth 2 m (3 m at DEJU) 1 m
Sampling Frequency per Site Once Once
Locations per Site One 10-34

Data Products

Megapit

A wide range of information about these soils is available in NEON data products, including:

  • Texture and bulk density: Soil physical properties (Megapit) (DP1.00096.001)
  • Soil pH, total carbon (C), nitrogen (N), sulfur (S) and other elements (Al, Ca, Fe, K, Mg, Mn, Na, P, Si, Sr, Ti, and Zr): Soil chemical properties (Megapit) (DP1.00097.001)
  • Root biomass distribution: Root sampling (Megapit) (DP1.00066.001)
  • Pedon description and photos

Soil samples with an “archiveID” in the “…perarchivesample…” file of the Soil physical properties (Megapit) or Soil chemical properties (Megapit) data products are available for request.

Distributed Initial Characterization

A wide range of information about these soils is available in NEON data products, including:

  • Texture and bulk density: Soil physical properties (Distributed initial characterization) (DP1.10047.001)
  • Soil pH, total carbon (C), nitrogen (N), sulfur (S) and other elements (Al, Ca, Fe, K, Mg, Mn, Na, P, Si, Sr, Ti, and Zr):  Soil chemical properties (Distributed initial characterization) (DP1.10008.001)

Previous Megapit Soil Archive Requests

Wondering how others are using NEON soil samples? Here is a list of previous requests. We encourage you to collaborate with these researchers if you have similar interests! 

Focus area: Humic and fulvic acids
Sites requested: All
Horizons requested: All
Years samples provided: 2013-2016
Requestor: Geoff Davies, Northeastern University
Project products:

  • https://dl.sciencesocieties.org/publications/sh/abstracts/56/6/sh15-07-0014
  • https://www.nrcs.usda.gov/wps/PA_NRCSConsumption/download?cid=nrcseprd374284&ext=pdf

Focus area: Bulk 14C content
Sites requested: All
Horizons requested: All
Years samples provided: 2015-2018
Requestor: Carlos Sierra, Max Planck Institute for Biogeochemistry


Focus area: Bulk 15N, 13C, oxalate and dithionite extractable Fe, Al, Si and oxalate and dithionite extractable Fe, Al, Si  on the silt and clay fraction
Sites requested: All
Horizons requested: All
Years samples provided: 2014-2018
Requestor: Marc Kramer, University of Florida
Project products: https://www.nature.com/articles/s41558-018-0341-4


Focus area: Oxygen isotope systematics during nitrification
Sites requested: HARV, OSBS, ORNL, TALL, CPER, JORN
Horizons requested: A (shallowest subhorizon)
Years samples provided: 2015
Requestor: Greg Michalski, Purdue University


Focus area: FTIR, mineral-associated OM, metal extractions
Sites requested: HARV, BLAN, SCBI, OSBS, JERC, DSNY, DELA, UNDE, KONZ, UKFS, DCFS, WOOD, NOGP, CPER, STER, RMNP, OAES, NIWO, JORN, ONAQ
Horizons requested: All mineral
Years samples provided: 2016-2018
Requestor: Jonathan Sanderman, Woods Hole Research Center
Project products
https://scisoc.confex.com/scisoc/2019am/meetingapp.cgi/Paper/121702


Focus area: Composition of soil C based on new solid state spectroscopic methods
Sites requested: All
Horizons requested: O and A (all subhorizons)
Years samples provided: 2017
Requestor: Margaret Bowman, University of Colorado


Focus area: Biogeography of Streptomyces
Sites requested: All
Horizons requested: A (shallowest subhorizon)
Years samples provided: 2018
Requestor: Steven Higgins, Cornell University


Focus area: The role of soil and iron-organic matter complexes as controls on redox chemistry driving NO2(g)-to-HONO conversion across a gradient of soil
Sites requested: HARV, SCBI, OSBS, GUAN, UNDE, KONZ, TALL, WOOD, CLBJ, NIWO, SRER, ONAQ
Horizons requested: A (shallowest subhorizon)
Years samples provided: 2018
Requestor: Rebecca Abney, Indiana University, Bloomington
Project products: https://agu.confex.com/agu/fm18/meetingapp.cgi/Paper/369558


Focus area: Carbon content of different soil fractions
Sites requested: All
Horizons requested: All
Years samples provided: 2019
Requestor: Jocelyn Lavallee, Colorado State University


Focus area: 13C nuclear magnetic resonance (CPMAS NMR) spectroscopic analyses to assess controls on soil organic matter chemical composition
Sites requested: HARV, SCBI, DSNY, OSBS, GUAN, UNDE, KONZ, GRSM, LENO, WOOD, CPER, NIWO, SRER, ONAQ, BART, JERC, DCFS, CLBJ, MOAB, JORN, RMNP, LAJA, UKFS, MLBS, ORNL, TALL, ABBY, WREF, SJER, SOAP, TEAK, TOOL, BONA, HEAL
Horizons requested: A (shallowest subhorizon)
Years samples provided: 2018
Requestor: Steven Hall, Iowa State University


Focus area: Quantitative guidelines for establishing and operating soil archives
Sites requested: NA (no samples required)
Horizons requested: NA (no samples required)
Years samples provided: NA
Requestor: Edward Ayres, NEON
Project products: https://acsess.onlinelibrary.wiley.com/doi/full/10.2136/sssaj2019.02.00…
 


Permit Requirements

All requesters must have a Permit to Receive Soil (PPQ 525) from the USDA Animal and Plant Health Inspection Service (USDA-APHIS), or from an equivalent international service. Find information about the permit at the APHIS website. Samples will not be shipped to facilities that do not have an active permit.

Considerations for Approval

In order to balance preservation and sample use, request only the minimum amount of soil needed for your study. Any amount of material may be requested; however, requests for smaller quantities of soil per sample (e.g., <20 g/Megapit sample or <10 g/ICS sample) are more likely to be approved. Requests for larger quantities may be approved if justified, the aim of the study passes the merit review, and sufficient material is available in the archive to support future requests. Do not request spare or extra soil. A minimum quantity of "forever sample" (10 g) will be reserved in the ICS archive for each sample for long-term keeping and potential re-analysis. All requests undergo a review process to evaluate the intellectual merit, quantity justification, and data management plan. Requests are evaluated to ensure that:

  • A legitimate research project is the basis for the request, including agency and award information if applicable;
  • The use of NEON soil samples is important to achieve the study goals;
  • The quantity is justified. Staff also review whether a sample was previously used for the same purpose; and
  • A specific data management plan describing how the data generated from the request will be made publicly available in a format suitable for reuse by others (e.g., see FAIR data principles). If possible, include links to publicly available data that you have previously generated to demonstrate a commitment to open science.

In addition, the evaluation will consider the impact of fulfilling the request on the samples’ projected exhaustion date, specifically whether they will be exhausted prior to NEON’s expected 2049 end date (megapit requests) or the ICS project end date (ICS requests), in order to maintain the ability to support future requests. This is achieved by extrapolating the sample consumption rate after fulfilling the proposed request into the future to determine the projected sample mass at the end date (Ayres et al. 2019). Given that some samples have a faster consumption rate than others and some samples had a lower original mass than others, NEON may approve a request for some samples but not others included in the request. Staff will typically reject requests for a specific sample in cases where fulfilling the request would result in that sample being exhausted prior to the end date. In this case, a requester may provide a justification explaining why that sample is essential to their project and the decision will be reevaluated.

In the event that a request is rejected or partially rejected, a requester may ask for a second evaluation by an external panel if they deem it necessary.

Request review procedure and timeline

The approval of requests for ≤20 g soil/Megapit sample is determined internally by NEON staff, while the approval of requests for ≤4 g (organic horizons) or  ≤15 g (mineral soils)/ICS samples is determined internally by NEON and UMBS-SAFE staff. A recommendation is sought from an external group of experts prior to determining an approval decision for requests above these thresholds. A first decision, which may be requesting additional information, is typically achieved within 1 week for requests reviewed internally and within 2 weeks for requests reviewed externally. 

Tips to avoid delays in the review of your request include:

  • Provide all requested information
  • Justify the quantity of soil requested for each analysis (include relevant citations where appropriate)
  • Avoid requests for soil analyses to be run in duplicate or triplicate, or if absolutely necessary, providing a suitable justification (include relevant citations where appropriate)

Request fulfillment

To maximize the representativeness of the subsample, requests for ≤1 g/megapit sample are fulfilled with finely ground soil generated with a ball mill, whereas larger requests are fulfilled with soil sieved through 2 mm mesh (mineral horizons) or hand sorted (organic horizons), unless otherwise requested.

Megapit soil samples are typically shipped within 2 months of request approval as long as permitting delays are not encountered. ICS samples are typically shipped within 1-4 months of request approval, depending on the number of samples requested, and provided permitting delays are not encountered.

In order to maintain the quality of the archived soils, returning subsamples to the archive after nondestructive analyses is not permitted due to the reduction in quality assurance that can be provided for material that has changed hands, as well as to protect the archives from external contaminants (e.g., isotopic tracers or biological contaminants). As a result, every subsampling event results in the permanent loss of soil from the archive.

Acknowledgement and Citation Policy

See NEON Data Usage and Citation Policies for details. 

Suggested Megapit Archive acknowledgement: "The National Ecological Observatory Network is a program sponsored by the National Science Foundation and operated under cooperative agreement by Battelle Memorial Institute. This material is based in part upon work supported by the National Science Foundation through the NEON Program, including samples provided by the NEON Megapit Soil Archive."

Suggested ICS Archive acknowledgement: "The National Ecological Observatory Network is a program sponsored by the National Science Foundation and operated under cooperative agreement by Battelle Memorial Institute. This material is based in part upon work supported by the National Science Foundation through the NEON Program, including samples from the NEON Distributed Plot Soil Archive, a joint project of NEON and the University of Michigan Biological Station, as supported by the National Science Foundation (DBI-1624205) and Battelle Memorial Institute (US001-0000757206)."

The following documents, available through the NEON document library, describe procedures related to the Megapit Soil Archive samples and can be cited as necessary:

  • Megapit Soil Archive field sampling and collection methods: Ayres E, Zulueta R (2013) TIS Soil Pit Sampling Protocol. NEON.DOC.001307;

  • Megapit Soil Archive sample processing: Ayres E and Loescher HW (2012) NEON Field and Lab Procedure and Protocol: TIS Soil Archiving. NEON.DOC.000325; and

  • Megapit Soil Archive requestor subsample processing: Ayres E (2013) NEON Procedure and Protocol: Producing TIS Soil Archive Subsamples for Users. NEON.DOC.001306.

The following documents, available through the NEON document library in the External Lab Protocols > NRCS Initial Soil Characterization folder, describe procedures related to the Initial Soil Characterization sampling effort and can be cited as necessary:

  • Browning, D. and L. Stanish. 2017. Guidelines for the NEON Soil Characterization Effort.

  • Schoeneberger, P.J., D.A. Wysocki, E.C. Benham, and Soil Survey Staff. 2012. Field book for describing and sampling soils, Version 3.0. Natural Resources Conservation Service, National Soil Survey Center, Lincoln, NE.

Additionally, the following paper outlines key guidelines and procedures that will be used in administering the soil archives:

  • Ayres, E. 2019. Quantitative guidelines for establishing and operating soil archives. Soil Science Society of America Journal 83:973-981. doi: 10.2136/sssaj2019.02.0050


Request Form

To complete this request form, you must include:

  • Your full name and affiliation
  • A copy of your USDA-APHIS permit to receive soil or equivalent permit
  • The archive you are requesting from (Megapit Archive or ICS Archive). Submit separate requests if requesting from both archives.
  • A project description, including:
    • Project objectives and background information;
    • A list of each analysis you will perform, description of how the analysis relates to the project objective, the quantity of soil required for each analysis, and a justification for the quantity used in each analysis;
    • A list of samples and the mass requested;
    • A detailed data management plan describing how data generated from the request will be made publicly available in a format suitable for reuse by others (e.g., see FAIR data principles). If possible, include links to publicly available data that you have previously generated to demonstrate a commitment to open science.
  • Agreement to the consent agreement (see below)

If you have questions, please email the Soil Archive Team (Megapit) or NEON and UMBS-SAFE staff (ICS).

Fields followed by an asterisk (*) are required.

Please the name of the institution or organization you are affiliated with.
Email *
Please attach a copy of your USDA-APHIS Permit to Receive Soils. Submit relevant national permit if located outside of the U.S.
One file only.
5 MB limit.
Allowed types: jpg, png, pdf.
Please attach a copy of your USDA-APHIS Compliance agreement to Receive Soils. Submit relevant national agreement if located outside of the U.S.
One file only.
5 MB limit.
Allowed types: jpg, png, pdf.
Archive Samples Being Requested

Please upload a description and justification for your request for NEON samples that includes:

  1. A brief project objective, description and justification for the use of NEON samples
  2. A list of analyses you will perform, the quantity of soil required for each analysis and a justification for the quantity used in each analysis
  3. A detailed list of requested samples. Do not request spare or extra soil material. 
Accepted file types include .doc, .docx and .pdf. Maximum upload size is 2MB.
One file only.
10 MB limit.
Allowed types: pdf, doc, docx.
Consent Agreement
By submitting a request, requestors agree to: 1) report all publications, presentations or other derived products resulting from the use of the samples to archive staff; 2) acknowledge NEON (Megapit Archive) or NEON and UMBS-SAFE (ICS Archive) as the provider of samples in any publication or presentation resulting from the request, regardless of authorship; 3) have your name, affiliation, and summary information about your request (e.g., focus area, sites and horizons requested) listed on the archive webpage in order to allow others with similar interests to know of your project; and 4) publish data generated from the request in a publicly available location in a format suitable for reuse by others (e.g., see FAIR data principles) within two years of receipt of the samples or or upon publication of results (whichever is shorter, with variances from this agreement approved based on a request in writing).  Please review NEON Data Usage and Citation Policies.
How did you hear about NEON's soil archive?
NEON Logo

Follow Us:

Join Our Newsletter

Get updates on events, opportunities, and how NEON is being used today.

Subscribe Now

Footer

  • My Account
  • About Us
  • Newsroom
  • Contact Us
  • Terms & Conditions

Copyright © Battelle, 2019-2020

The National Ecological Observatory Network is a major facility fully funded by the National Science Foundation.

Any opinions, findings and conclusions or recommendations expressed in this material do not necessarily reflect the views of the National Science Foundation.